Mutants of Alcaligenes eutrophus defective in autotrophic metabolism
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چکیده
منابع مشابه
Cyclic nature of poly(3-hydroxyalkanoate) metabolism in Alcaligenes eutrophus.
The cyclic process of biosynthesis and degradation of poly(3-hydroxyalkanoate) (PHA) was studied in Alcaligenes eutrophus under conditions of nitrogen-limitation of growth. A. eutrophus cells, which had accumulated poly(3-hydroxybutyrate) (PHB) of 55 wt% content within cells from butyric acid, were transferred into a nitrogen-free medium containing pentanoic acid as the sole carbon source and c...
متن کاملUnusual C3 and C4 metabolism in the chemoautotroph Alcaligenes eutrophus.
Phosphoenolpyruvate (PEP) carboxykinase was identified to be the only C3-carboxylating enzyme in Alcaligenes eutrophus. The enzyme requires GDP or inosine diphosphate (GTP or inosine triphosphate) for activity. Pyruvate- and other PEP-dependent CO2-fixing enzyme activities were not detected, regardless of whether the cells were grown autotrophically or heterotrophically. It is suggested that tw...
متن کاملDissimilation of aromatic compounds by Alcaligenes eutrophus.
The range of aromatic compounds that support the growth of Alcaligenes eutrophus has been determined, and the pathways used for the dissimilation of these substrates have been explored, largely by enzymatic analyses. The beta-ketoadipate pathway operates in the dissimilation of benzoate and p-hydroxybenzoate; the genetisate pathway, in the dissimilation of m-hydroxybenzoate; and the meta cleava...
متن کاملDenitrification by Alcaligenes eutrophus is plasmid dependent.
Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of converting some nitrate to nitrite, but they did not metabolize nitrite under anaerobic...
متن کاملAlcaligenes eutrophus as a bacterial chromate sensor.
In Alcaligenes eutrophus CH34, determinants encoding inducible resistance to chromate (chr) and to cobalt and nickel (cnr) are located adjacent to each other on plasmid pMOL28. To develop metal-sensing bacterial strains, a cloned part of plasmid pMOL28, which contains both determinants, was mutated with Tn5-lacZ. The chr::lacZ fusions were specifically induced by chromium; cnr was induced best ...
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ژورنال
عنوان ژورنال: Archives of Microbiology
سال: 1978
ISSN: 0302-8933,1432-072X
DOI: 10.1007/bf00402299